Free: we analyse your single-cell data, not just QC it

The file type is detected automatically: single-cell, Visium spatial and bulk count matrices each get the quality metrics that belong to them — a spot is not a cell, and a doublet estimate on spatial data would be meaningless.

Cannot share the data? Use the no-upload self-check — five numbers from the Cell Ranger summary, computed in your browser.

Upload a count matrix and get back the QC every tool gives you — per-cell distributions, data-driven thresholds (median ± 3 MAD, not copied from a tutorial), a barcode-rank plot and the genes that dominate your counts — and then the analysis itself: clustering, a UMAP and automated cell-type annotation of your own data, typically within two minutes. No account, no email required. Your file is deleted once the report is built — in practice a couple of minutes after the analysis

Cannot share the file? Type five numbers instead — assessed in your browser, nothing uploaded.
No data to hand? Open an example report — the same output, run on the public 10x PBMC 3k dataset.

bulk count matrix too — if the file turns out to be a bulk RNA-seq matrix (a few dozen samples rather than thousands of cells) we detect that and run the bulk checklist instead: library sizes, detected genes, sample correlation, PCA outliers and a gene-filtering rule.

Runs on our own machines, typically 30 seconds to 3 minutes depending on size. Nothing is stored: the input is removed as soon as the report is generated, and the report itself expires after 7 days.

What you get

Per-cell distributions

Genes, UMIs and mitochondrial fraction per cell, with the suggested cut-offs drawn on top.

Thresholds that fit your data

Computed from your own distributions rather than the standard 200 / 2500 / 5% that fails on many tissues.

Warnings worth acting on

Missing mitochondrial annotation, undetected empty droplets, suspiciously shallow profiles, over-aggressive filtering.

Clustering and annotation

Not just QC: your data clustered, embedded and labelled by cell type — the same pipeline that starts a paid project, run on a subsample so it finishes while you wait.

A PDF you can forward

Everything above as a document for your supervisor or core facility. No attribution required.

→ Why fixed QC thresholds fail, and what to use instead